Specific Binding Protein for Active Metabolites of Vitamin D3

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Specific binding protein for active metabolites of vitamin D3.

FIG. 1 Separation of the specific protein binding with vitamin D3 and its metabolites in rat intes tinal cytoplasm by gel filtration on Sephadex G 200 after the administration of vitamin D3-3H in vivo. 1,000I.U. (3•~105cpm) of randomly labeled vitamin D3 (sp. act. 1.2•~104cpm/ug) was injected intracar dially to vitamin D deficient rat 16 hours before decapitation. 2.0 ml of rat intestinal cytop...

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Effects of vitamin D3 and calcium supplementation on serum levels of tocopherols, retinol, and specific vitamin D metabolites.

γ-Tocopherol (γT) protects against DNA-damaging effects of nitrogen oxides, yet its physiologic regulation in vivo is unknown. Observational studies indicate inverse associations of 25[OH]-vitamin D with γT and leptin. To determine whether vitamin D(3) supplementation alters levels of lipid-soluble micronutrients, serum samples (N = 85 subjects) from a randomized, double-blind, placebo-controll...

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Diurnal rhythms of vitamin D binding protein and total and free vitamin D metabolites

Vitamin D binding protein (DBP) concentration is known to influence the availability and bioactivity of vitamin D metabolites but its diurnal rhythm (DR), its inter-relationships with the DRs of vitamin D metabolites and its influence on free vitamin D metabolite concentrations are not well described. The DRs of plasma total 25(OH)D, total 1,25(OH)2D, DBP, albumin and calculated free 25(OH)D an...

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Enhancement of vitamin D metabolites in the eye following vitamin D3 supplementation and UV-B irradiation.

PURPOSE This study was designed to measure vitamin D metabolites in the aqueous and vitreous humor and in tear fluid, and to determine if dietary vitamin D3 supplementation affects these levels. We also determined if the corneal epithelium can synthesize vitamin D following UV-B exposure. METHODS Rabbits were fed a control or vitamin D3 supplemented diet. Pilocarpine-stimulated tear fluid was...

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Lysine-specific gingipain promotes lipopolysaccharide- and active-vitamin D3-induced osteoclast differentiation by degrading osteoprotegerin.

Porphyromonas gingivalis is one of the major pathogens of periodontitis, a condition characterized by excessive alveolar bone resorption by osteoclasts. The bacterium produces cysteine proteases called gingipains, which are classified according to their cleavage-site specificity into Kgps (lysine-specific gingipains) and Rgps (arginine-specific gingipains). In the present study we examined the ...

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ژورنال

عنوان ژورنال: THE JOURNAL OF VITAMINOLOGY

سال: 1971

ISSN: 0022-5398

DOI: 10.5925/jnsv1954.17.119